Biliverdin Reductase Protein (AA 3-296)
Quick Overview for Biliverdin Reductase Protein (AA 3-296) (ABIN666791)
抗原
See all Biliverdin Reductase (BLVRA) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 3-296
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序列
- MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK
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产品特性
- Biliverdin reductase A, 3-296 aa, Human, Recombinant, E.coli
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.
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Biliverdin Reductase A (BLVRA) (AA 1-341) protein (His-IF2DI Tag)
BLVRA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Biliverdin Reductase A (BLVRA) (AA 1-296) protein (GST tag)BLVRA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Biliverdin Reductase A (BLVRA) protein (Myc-DYKDDDDK Tag)BLVRA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))
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别名
- Biliverdin Reductase A/BLVRA
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背景
- BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. ( Bilirubin + NAD (P) + = biliverdin + NAD (P) H ) Recombinant BLVRA protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 33.3 kDa (295aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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NCBI登录号
- NP_000703
抗原
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